Re: Josh's comment on Rokas vs. Wells

From: Denyse O'Leary (oleary@sympatico.ca)
Date: Sat Oct 25 2003 - 08:24:21 EDT

  • Next message: Jack Haas: "Re: Wells and Molecular Phylogenies"

    I was interested in this item, and showed it to a net buddy, who then
    asked me to get the list's thoughts on this:

    "Josh Bembenek thought that the article by Rokas et al., "Genome-scale
    approaches to resolving incongruence in molecular phylogenies" (NATURE
    425[23 October 2003], 798 - 804) would make Jonathan Wells's critique of
    molecular phylogenies "much harder to sustain."

    "I've read Wells's 2000 book, ICONS OF EVOLUTION, as well as the NATURE
    article by Rokas et al., and two things occur to me: First, Rokas et al.
    actually confirm something Wells wrote three years ago. Wells wrote
    that the molecular data at that time were not converging as expected on
    consistent patterns because "different molecules lead to different
    phylogenetic trees," and "the problems extend even to smaller branches,
    including animal phylogenies." Now Rokas et al. write in the first
    paragraph of their article: "Despite tremendous progress in recent
    years, phylogenetic reconstruction involves many challenges that create
    uncertainty with respect to the true historical associations of the taxa
    analysed. One of the most notable difficulties is the widespread
    occurrence of incongruence between alternative phylogenies generated
    from single-gene data sets. Incongruence occurs at all taxonomic
    levels, from phylogenies of closely related species to relationships
    between major classes or phyla and higher taxonomic groups."

    "As I recall, Wells's main point was that it is misleading to give
    biology students the impression that molecular studies have confirmed
    Darwin's tree of life, when in fact those studies are plagued by
    inconsistencies. Judging from the 2003 article by Rokas et al., Wells
    was certainly correct in his assessment of the situation in 2000.

    "Second, Rokas et al. think they have found a way to overcome the
    incongruence problem -- i.e., by relying on concatenations of 20 or more
    genes. They demonstrate that they can do this within the yeast genus
    Saccharomyces. Can the same method overcome incongruences at higher
    taxonomic levels? Rokas et al. are optimistic; their concluding
    sentence is: "The results of our study suggest that use of genome-wide
    data sets may provide unprecedented power not only in testing specific
    phylogenetic hypotheses but also in precise reconstruction of the
    historical associations of all the taxa analysed."

    "If the method of using genome-wide data sets does, in fact, resolve the
    incongruence problem generally, then it will indeed be harder (if not
    impossible) to argue that molecular studies fail to support evolutionary
    theory. Until the method is actually applied to higher taxonomic
    levels, however, we don't know whether it will work at those levels.
    Maybe it will, and maybe it won't. What if it turns out that a minimum
    of 20 genes will suffice for a genus-level analysis, but 200 genes are
    needed for an order-level analysis, 2000 for a class-level analysis, and
    20000 for a phylum-level analysis? Will it even be practical to analyze
    such numbers? We don't know. And until we DO know, isn't it better (as
    Wells argued three years ago) NOT to give students the impression that
    molecular phylogenies have confirmed Darwin's tree of life? This is not
    to say that the tree of life has been DIS-confirmed, but only that we
    shouldn't claim more for the evidence than it actually shows."

    Any comments?

    Denyse

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